Monoclonal antibodies

The production of monoclonal antibodies, using the hybridoma methodMonoclonal antibodies atomic number 18 obtained from clones of single B cells. Unfortunately, B cells go out not erect in gardening and this problem has to be got round by fusing them withmalignant B myeloma cells i.e. malignant B cells. Myeloma cells will continue to fix and single out indefinitely, though they do not erect antibodies. The f rehearsed cellsproduced from myeloma cells with B cells be known as hybridomas. The hybridoma cells will also continue to grow and appoint (given suitable and enough nutrients) andthey do exude antibodies. The antibodies that they secrete argon the specific antibodies that were produced by the original clone of B cells. The production of monoclonal antibodies involves the quest stages :? A mo pulmonary tuberculosis is injected with antigen for which theantibodies are required? An immune reaction takes place and the mouse plasmacells set off to puzzle head the ant ibody? Plasma cells are extracted from the mouse? The plasma cells are f utilise with B cell myeloma cells? The resulting hybridoma cells are separated individu eachyand eachowed to grow, divide and produce antibodies? Some antibodies are removed and tested with the relevantantigen, to make sure they are the correct monclonals? Those hybridoma cells which are producing the requiredantibodies are finale in a large fermenter? The monoclonal antibodies are harvested and purifiedThe use of monoclonal antibodies in the diagnosis and treatment of disease and motherliness testingDiagnosis ? because the monoclonal antibodies produced from a clone of B cells are all identical, they can be utilise to identify macromolecules with a little high degree ofspecificity. For example, they are now routinely used for the following:? Blood type before transfusions? Tissue typing before transplants? Identification of pathogens ? using monoclonals, it is now possible to denounce between diffe rent strains of certain pathogens, which wou! ld, otherwise, be every insensitive? Identification... If you want to get a full essay, order it on our website: OrderCustomPaper.com

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